Enzyme 10x exclusively developed for hospitals, We observed majority of hospitals laundries not providing hot water facility, Even they don't understand hot water value in hospitals laundry, Maximum stains will go if they provide hot water, Here Enzyme 10x replacement who are not maintain hot water, Multiple Carbon Based Enzymes when desolved in water automatically start catalyst function, Will dissolve protein stains like blood, and motions etc With out hot wwater also it will works and in hard water also Enzyme 10x works but use in main wash along with our Emulsifier Active 250mg because if you add Bleach with this detergent that will damage enzymes efficacy !!! Kindly follow our protocol For more information Contact Nagaraju. M C.E.O Mylab Biotech Chemicals 9392561829